Detection and Inhibition of Staphylococcus aureus Biofilms by Chemical Agents

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Fizza Nazim
Maria Khan
Namra Usman
Mehsa Hashim
Eisha Hanan
Shayan Ali Shah
Sohaib Irfan
Khalid Ahmed
Syed Hani Abidi


INTRODUCTION: Staphylococcus aureus, a gram-positive pathogen, is one of the most frequent causes of biofilm-associated infections on indwelling medical devices. Biofilm is formed when bacteria live in communities and form a matrix as a survival mechanism in a generalized manner. With the emergence of methicillin-resistant Staphylococcus aureus (MRSA) and its biofilm-forming ability there is an urgent need to discover active agents against Staphylococcus aureus. One possible way to control biofilm formation on indwelling medical devices is to coat the devices with the agents that can inhibit bacterial colonization and biofilm formation. To test this concept, in this study, we used several assays to detect biofilm forming potential of a clinical isolate of Staphylococcus aureus, followed by testing of the ani-biofilm activity of three chemical agents.


materials and METHODs: Staphylococcus aureus clinical isolate was screened for susceptibility/resistance against eight commonly prescribed antibiotics (ciprofloxacin, chloramphenicol, ampicillin, amikacin, cephalothin, clindamycin, streptomycin and gentamicin) using the Kirby-Bauer disk diffusion method. We applied both qualitative and quantitative assays to detect the biofilm formation by the Staphylococcus aureus isolate. In the next step, antibiofilm activity of three chemical agents, namely SDS, NaOH and Tween20, was examined using qualitative and quantitative biofilm reduction assays.


RESULTS: The Staphylococcus aureus isolate was found to be susceptible to all antibiotics except clindamycin. The individual Minimum Inhibitory Concentration (MIC) of SDS, NaOH, and Tween20 against Staphylococcus aureus isolate was found to be 0.05%, 1M and 5%, respectively. In biofilm reduction assay, we found a noteworthy biofilm inhibition potential of these agents, which was 89.41% for Tween20, 83.05% for SDS and 71.93% for NaOH.

 CONCLUSIONS: These compounds are generally used in laboratories for diverse research purposes. These chemicals can be repurposed for their ability to inhibit the biofilm produced by Staphylococcus aureus, hence providing an effective alternative to deal with the problem of catheter-associated infections.

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